Hungate Tubes

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Printed Combined Screw-Cap Rubber-Stopper Closure Hungate ...
For Hungate Tubes (Pre-reduced Anaerobically Sterilized Roll Tubesand Liquid Media) H. R. ATTEBERY AND S. M. FINEGOLD quired for inoculating a large number of tubes would make the practice impractical. A screw-capped closure should be made available in an ... View Document

Cultivation Of Anaerobes - DSMZ: Home
Cultivation of Anaerobes We recommend to use the Hungate technique to culture these The Hungate-type tubes are closed with a flange-type butyl rubber septum and a screw cap with 9 mm opening to allow puncturing of the septum with injection needles. ... Get Doc

Brain Heart Infusion (Broth Media) Brain Heart Infusion ...
BBL™ Brain Heart (Infusion) CC Agar Cat No. 211057 Dehydrated – 500 g 296261 Prepared Plates (Deep Fill) – Pkg. of 20* pre-reduced in Hungate tubes is recommended for the cultivation of anaerobic microorganisms, particularly obligate anaerobes. ... Fetch Doc

1 - Techniques For Cultivating Methanogens
Techniques for Cultivating Methanogens Ralph S. Wolfe Contents 1. Introduction 1 2. Methanogens on a Budget 2 Growth of methanogens in a Hungate roll tube 11 2.7. Preparation of a 3-L amount of anoxic medium 12 Assemble 20 aluminum seal anaerobic culture tubes or 20, 40 mL serum ... Retrieve Doc

SUPPLEMENTARY NOTE 1 Cultures Used In This Study
Hungate tubes containing RM02 + GenRFV25 oand incubated at 39 C. Tubes that showed any turbidity were scored as positive for growth, subcultured into fresh RM02 media, examined microscopically for cell uniformity and morphology and stored at -85 o C. ... Return Document

Thiosulfate Reduction And Alanine Production In Glucose ...
Thiosulfate reduction and alanine production in glucose fermentation by experiments were performed in unstirred 15 ml Hungate tubes with 10 ml of medium. Samples were taken after 14 days of incubation at 55 C, the results are means of two parallel runs. The standard ... Retrieve Doc

A Quick Method For The Determination Of Dissolved And ...
Cooled under N, as the gas phase (Hungate technique [5, 61) and the vessel was sealed with a rubber septum. Five ml of this ahoxic water was dispensed by syringe into several 14 ml N, filled, septum sealed test tubes (Hungate tubes, Bellco Glass Inc., Vineland, NJ, USA). A washed crystal of Na$. ... Get Content Here

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Effect Propionate Toxicity Methanogen-Enriched Sludge ...
Replicate Hungate tubes containing Balch medium3 with 0to 200 mMsodium propionate, prepared as described above, were inoculated with 0.5 ml of7-day cultures ofMethanobrevibactersmithii and Methanospirillum hungatii. The inoculated tubes were incubatedat 37°Candagitated as described above. Methane production in the headspace and the optical ... Read More

The Biology Of Anaerobic Bacteria And Predominant Propagation ...
The Biology of Anaerobic Bacteria and Predominant Propagation Practices Allison Faust Senior Biologist, ATCC •Hungate tubes •Balch tubes 14. Inhibitors Macy JM, Snellen JE, and Hungate RE. Use of syringe methods for anaerobiosis. The American Journal of Clinical Nutrition 25: 1318- ... Return Doc

A) Green PhDtotrophic Bacteria Isolated From A Winograsky ...
Of these enrichments were inoculated (10%) into four hungate tubes: tube A contains 10 ml marine sulfate reducer medium plus5 mM acetate 1% CO in the headspace (0.17 ml 100% CO/ 17 ml headspace); tube B contains 10 ml s.r. medium plus 5 mM acetate; tube C contains 10 ml s.r. medium plus 1% CC in the headspace and tube D contains 10 ml s.r. medium ... Access Document

Brain Heart Infusion (Broth Media) Brain Heart Infusion ...
Before inoculating Hungate tubes, disinfect the septum of the cap. To inoculate, insert needle of syringe containing specimen through the septum and inject the specimen into the medium. Withdraw the needle slowly to avoid introducing air into the tube. ... Fetch This Document

Axial Ripsaws On XR10 - YouTube
Brian Parker tests Axial's new 2.2 Ripsaws on his XR10-a-licious. ... View Video

A Serum Bottle Modification Of The Hungate Technique For ...
Hungate Technique for Cultivating A Serum Bottle Modification of the to our specifications, 18 by 150-mm test tubes with a serum bottle neck (Wheaton Scientific blueprint no. 07350) (Fig. 2B). ... Return Doc

Novel Strains Of Moorella Thermoacetica Form Unusually Heat ...
And 121°C, respectively, by direct immersion of inverted Hungate tubes (ensuring that the rubber stoppers were covered). Duplicate tubes containing media with inserted thermometers were used to ascertain lag time and desired temperature. ... Fetch Content

Special Instructions - Leibniz-Institut DSMZ
The Hungate-type tubes are closed with a flange-type butyl rubber septum and a screw cap with 9 mm opening to allow puncturing of the septum with injection needles. Balch-type tubes are more stable than Hungate-type tubes and recommended if an overpressure of 2 to 3 bar can be expected during culturing. ... Document Retrieval

Evidence Of Two Oxidative Reaction Steps Initiating Anaerobic ...
Were performed under strictly anoxic conditions at 30°C in 5-ml Hungate tubes or 1.5-ml cuvettes, using anoxic buffers and solutions. The tubes and cuvettes were flushed with N 2 and closed with butyl septa. All additions and samplings were done with gastight Unimetrix microliter syringes (Macherey-Nagel, Du¨ren, * Corresponding author. ... View Full Source

Mink, Ronald; Dugan, Patrick R. - Ohio State University
Hungate tubes were inoculated by the syringe variation of the technique of Macy et al (1972) with 1 ml of mixed culture inoculum. Mixed culture tubes were not gassed again after the initial gassing with CO2+N2. The mixed cul-ture was used for inhibition and stimulation studies with complete MS medium prepared with the appropriate carbohydrate ... Read More